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Updated: Apr 25, 2026
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Published on: November 25, 2015
Excitatory and inhibitory tonotopic bands in chinchilla inferior colliculus revealed by c-fos immuno-labeling
Lisa M D'Alessandro1, Robert V Harrison2
1Department of Physiology, University of Toronto, Toronto M5S 1A8, Canada; Institute of Biomaterials & Biomedical Engineering, University of Toronto, Toronto M5S 3G9, Canada; The Auditory Science Laboratory, Program in Neurosciences and Mental Health, The Hospital for Sick Children, Toronto M5G 1X8, Canada.
Abstract:
We describe in detail a reliable experimental protocol for c-fos immuno-labeling of patterns of neural activation in the chinchilla (chinchilla laniger). We report on resting-level neural activity in inferior colliculus (IC) of auditory midbrain, and on tonotopic bands present following 90 min of pure-tone sound stimulation. Neurons activated by 6-kHz sound stimulation lay ventro-medial to those activated at 2 kHz. This is consistent with the known tonotopic organization of IC, and verified in the present report by multi-unit neuron response recordings in central nucleus of IC. Of particular interest, we observe a significant reduction in cell labeling adjacent to the tonotopic bands, and suggest that such decreases represent inhibitory regions. C-fos-labeled bands and lateral regions of reduced labeling resemble excitatory and lateral-inhibitory response areas of IC neurons.
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