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A rapid method for isolation of human hemoglobin benzo[a]pyrene diol epoxide derived adducts using high performance
1Department of Chemistry, Massachusetts Institute of Technology, Cambridge 02139.
Biomedical Chromatography : BMC
|November 1, 1989
Abstract:
A method is described to isolate rapidly human hemoglobin-benzo[a]pyrene diol epoxide adducts. A combination of 300 A pore size C4 reversed phase HPLC to effect separation of adducted protein from native protein, and mu-bore C18 reversed phase HPLC to isolate and partially characterize proteolytic peptide adducts (by UV), was used.