Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

NF-κB-dependent Signaling Pathway02:26

NF-κB-dependent Signaling Pathway

7.4K
The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...
7.4K
NF-kB-dependent Signaling Pathway02:26

NF-kB-dependent Signaling Pathway

2.0K
2.0K
Regulation of Nuclear Protein Sorting01:45

Regulation of Nuclear Protein Sorting

2.5K
Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
2.5K
Co-activators and Co-repressors02:04

Co-activators and Co-repressors

6.9K
Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
6.9K
Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

8.2K
Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein....
8.2K
Receptor Downregulation in MVBs01:15

Receptor Downregulation in MVBs

2.0K
Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that  lead to cell proliferation, migration, and differentiation. Overexpression of EGFR  stimulates cells to proliferate. Excessive  EGFR...
2.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Selective H2-O Tissue Expression Reduces Risk for Graft-versus-Host Disease in an In Vivo Transplantation Model.

Transplantation and cellular therapy·2025
Same author

Mathematical birth of Early Afterdepolarizations in a cardiomyocyte model.

Mathematical biosciences·2023
Same author

Dietary fatty acids and endometrial cancer risk within the European Prospective Investigation into Cancer and Nutrition.

BMC cancer·2023
Same author

LIPSS-based functional surfaces produced by multi-beam nanostructuring with 2601 beams and real-time thermal processes measurement.

Scientific reports·2021
Same author

mTORC1 - a potential player in the pathogenesis of hidradenitis suppurativa?

Journal of the European Academy of Dermatology and Venereology : JEADV·2021
Same author

[Low-risk community-acquired pneumonia: implications of its outcome due to the determining factors for admission not contemplated by the scores].

Semergen·2019
Same journal

Mitochondrial-endoplasmic reticulum interactions in lung diseases.

Cell death & disease·2026
Same journal

A ZDHHC3-USP5-PTRF axis links palmitoylation to ferroptosis-associated phenotypes in melanoma.

Cell death & disease·2026
Same journal

Small molecule-mediated activation of Notch signal transduction.

Cell death & disease·2026
Same journal

OT-55 reshapes tolerogenic BH3-mimetic-induced apoptosis toward immunogenic cell death in acute myeloid leukemia, potentiating PD-1/Tim-3 blockade.

Cell death & disease·2026
Same journal

FOXQ1 activates GLT8D2 to enhance CCL2 N-glycosylation and promote prostate cancer bone metastasis.

Cell death & disease·2026
Same journal

PCGF1-mediated bivalent promoter remodeling enables NK cell immune evasion in non-small cell lung cancer.

Cell death & disease·2026
See all related articles

Related Experiment Video

Updated: Apr 25, 2026

A Chromatin Immunoprecipitation Assay to Identify Novel NFAT2 Target Genes in Chronic Lymphocytic Leukemia
09:52

A Chromatin Immunoprecipitation Assay to Identify Novel NFAT2 Target Genes in Chronic Lymphocytic Leukemia

Published on: December 4, 2018

7.2K

The RING ubiquitin E3 RNF114 interacts with A20 and modulates NF-κB activity and T-cell activation.

M S Rodriguez1, I Egaña2, F Lopitz-Otsoa2

  • 1Cancer Unit, Inbiomed, San Sebastian, Gipuzkoa, Spain.

Cell Death & Disease
|August 29, 2014
PubMed
Summary
This summary is machine-generated.

The study identifies RING-type zinc-finger protein 114 (RNF114) as a new regulator of nuclear factor-κB (NF-κB) signaling. RNF114 stabilizes key proteins, controlling T-cell activation and immune responses.

More Related Videos

NF-κB-dependent Luciferase Activation and Quantification of Gene Expression in Salmonella Infected Tissue Culture Cells
10:57

NF-κB-dependent Luciferase Activation and Quantification of Gene Expression in Salmonella Infected Tissue Culture Cells

Published on: January 12, 2020

9.9K
A Guide to Production, Crystallization, and Structure Determination of Human IKK1/α
11:27

A Guide to Production, Crystallization, and Structure Determination of Human IKK1/α

Published on: November 2, 2018

7.8K

Related Experiment Videos

Last Updated: Apr 25, 2026

A Chromatin Immunoprecipitation Assay to Identify Novel NFAT2 Target Genes in Chronic Lymphocytic Leukemia
09:52

A Chromatin Immunoprecipitation Assay to Identify Novel NFAT2 Target Genes in Chronic Lymphocytic Leukemia

Published on: December 4, 2018

7.2K
NF-κB-dependent Luciferase Activation and Quantification of Gene Expression in Salmonella Infected Tissue Culture Cells
10:57

NF-κB-dependent Luciferase Activation and Quantification of Gene Expression in Salmonella Infected Tissue Culture Cells

Published on: January 12, 2020

9.9K
A Guide to Production, Crystallization, and Structure Determination of Human IKK1/α
11:27

A Guide to Production, Crystallization, and Structure Determination of Human IKK1/α

Published on: November 2, 2018

7.8K

Area of Science:

  • Immunology
  • Molecular Biology
  • Cell Signaling

Background:

  • Nuclear factor-κB (NF-κB) activity requires precise regulation to prevent immune and inflammatory disorders.
  • NF-κB activation leads to the expression of negative regulators like IκBα and A20, which control the NF-κB pathway.
  • Dysregulation of NF-κB signaling is implicated in various diseases.

Purpose of the Study:

  • To identify novel interacting partners of A20 involved in NF-κB regulation.
  • To investigate the role of the identified factor in modulating NF-κB activity and T-cell responses.
  • To elucidate the mechanism by which this factor influences T-cell activation and apoptosis.

Main Methods:

  • Yeast two-hybrid screening to identify A20-interacting proteins.
  • Co-immunoprecipitation and ubiquitylation assays to study protein interactions and modifications.
  • Reporter assays to assess NF-κB-dependent transcriptional activity.
  • Analysis of T-cell activation, apoptosis, and cell cycle regulation.

Main Results:

  • The RING-type zinc-finger protein 114 (RNF114) was identified as an A20-interacting factor.
  • RNF114 interacts with A20 in T cells and modulates A20 ubiquitylation.
  • RNF114 negatively regulates NF-κB-dependent transcription by stabilizing both A20 and IκBα proteins.
  • RNF114 influences T-cell activation and apoptosis, independent of cell cycle regulation.

Conclusions:

  • RNF114 is a novel A20-binding protein that negatively regulates NF-κB signaling.
  • RNF114 plays a significant role in controlling T-cell activation and apoptosis.
  • This finding contributes to understanding the molecular mechanisms governing T cell-mediated immune responses.