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Modern Molecular Taxonomy

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Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...

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Updated: Apr 25, 2026

Sexual Transmission of American Trypanosomes from Males and Females to Naive Mates

Sexual Transmission of American Trypanosomes from Males and Females to Naive Mates

Published on: January 27, 2019

Optimized multilocus sequence typing (MLST) scheme for Trypanosoma cruzi.

Patricio Diosque¹, Nicolás Tomasini¹, Juan José Lauthier¹

¹Unidad de Epidemiología Molecular (UEM), Instituto de Patología Experimental, CONICET- Universidad Nacional de Salta, Salta, Argentina.

Plos Neglected Tropical Diseases

|August 29, 2014

Summary

This summary is machine-generated.

An optimized Multilocus Sequence Typing (MLST) scheme for Trypanosoma cruzi (T. cruzi) was developed. This new method efficiently identifies genetic lineages and genotypes of the Chagas disease agent, offering a potential new gold standard for typing.

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Ready-To-Use qPCR for Detection of DNA from Trypanosoma cruzi or Other Pathogenic Organisms

Ready-To-Use qPCR for Detection of DNA from Trypanosoma cruzi or Other Pathogenic Organisms

Published on: January 20, 2022

Area of Science:

Molecular Biology
Parasitology
Genetics

Background:

Trypanosoma cruzi exhibits significant genetic diversity, complicating accurate typing.
Existing typing methods like multilocus enzyme electrophoresis are laborious and time-consuming.
Whole genome sequencing is not yet feasible for large-scale T. cruzi typing.

Purpose of the Study:

To develop and optimize a Multilocus Sequence Typing (MLST) scheme for Trypanosoma cruzi.
To improve the accuracy and efficiency of identifying T. cruzi genetic lineages and genotypes.
To establish a potential gold standard for T. cruzi typing.

Main Methods:

Combined two proposed MLST approaches using thirteen concatenated gene fragments.
Applied the scheme to a panel of T. cruzi reference strains representing all known genetic lineages.
Assessed all locus combinations to select an optimized MLST scheme based on discriminatory power and bootstrap support.

Main Results:

The thirteen-fragment scheme successfully assigned strains to Discrete Typing Units (DTUs), with one outlier strain resolved by fragment exclusion.
An optimized seven-fragment MLST scheme discriminated all reference strains with robust bootstrap support.
A reduced four-fragment panel effectively assigned DTUs and discriminated 21 out of 25 genotypes.

Conclusions:

The optimized seven-fragment MLST scheme is proposed as a new gold standard for T. cruzi typing.
This scheme offers improved accuracy and efficiency compared to existing methods.
It provides a reliable basis for comparing other T. cruzi typing approaches.