Related Experiment Video
Updated: Apr 25, 2026

Chemoselective Modification of Viral Surfaces via Bioorthogonal Click Chemistry
Published on: August 19, 2012
A cleavable azide resin for direct click chemistry mediated enrichment of alkyne-labeled proteins
Christian Sibbersen1, Lennart Lykke, Niels Gregersen
1Department of Chemistry, Aarhus University, Langelandsgade 140, 8000 Aarhus C, Denmark. kaj@chem.au.dk.
Abstract:
The direct conjugation of a labeled proteome to a cleavable azide resin utilizing the copper-catalyzed azide alkyne cycloaddition is demonstrated. The procedure omits the classical streptavidin- and biotin-based affinity enrichment step and represents an operationally simpler, cheaper and less contaminated alternative for protein purification.
More Related Videos
09:06Efficient and Site-specific Antibody Labeling by Strain-promoted Azide-alkyne Cycloaddition
Published on: December 23, 2016
07:39Cell-Type Specific Protein Purification and Identification from Complex Tissues Using a Mutant Methionine tRNA Synthetase Mouse Line
Published on: April 13, 2022