Divergence of the nucleotide sequences encoding xanthine dehydrogenase in Calliphora vicina and Drosophila...

We present here two nucleotide sequences, from two different alleles encoding xanthine dehydrogenase in Calliphora vicina. One sequence covers the first exon with 1529 bp upstream from the initial ATG and 1737 bp downstream from the donor end of the first intron. The other sequence starts 2537 bp upstream from the acceptor site of the first intron, and ends 662 bp downstream from the putative polyadenylation site of the transcript. Comparison with the homologous gene from Drosophila melanogaster (rosy) reveals extensive divergence, with differences in the splicing patterns and no detectable homology between introns or flanking regions. Nevertheless, there is 76% identity between the amino acid (aa) sequences. The pattern of aa differences has been analysed and correlated with predicted three-dimensional (3-D) parameters. These studies consistently suggest that the evolution of the protein was strongly biased for conservation of its 3-D structure. Possible functional significance of the aa changes is discussed.