[The effect of thiol compounds and iron on formation of nitric oxide from nitroprusside and nitroglycerin]

Some thiol-containing substances 5-Na-thiosulfate, cysteine, reduced glutathione and reducing agent Na-dethionite were shown to elevate formation of nitric oxide from both Na-nitroprusside and nitroglycerol in water solutions, especially if Fe2+ was added into solution of nitroglycerol. Nitric oxide developed was maintained in solutions in the complexes containing water soluble dinitrosyl iron and thiol derivatives. Release of nitric oxide from nitroprusside and nitroglycerol in animals was caused by similar reactions. At the same time, nitric oxide, derived from nitroprusside, was involved in complexes containing dinitrosyl iron and a protein with double thiol group, whereas nitric oxide, obtained from nitroglycerol, was bound with hemoglobin, producing Hb-nitrosyl complexes.