Related Experiment Video
Updated: Apr 25, 2026

A Semi-High-Throughput Adaptation of the NADH-Coupled ATPase Assay for Screening Small Molecule Inhibitors
Published on: August 17, 2019
Monitoring ATP hydrolysis and ATPase inhibitor screening using (1)H NMR
Bingqian Guo1, Pinar S Gurel, Rui Shu
1Department of Chemistry, Dartmouth College, Hanover 03755, USA. maria.pellegrini@dartmouth.edu dale.f.mierke@dartmouth.edu.
Abstract:
We present a versatile method to characterize ATPase and kinase activities and discover new inhibitors of these proteins. The proton NMR-based assay directly monitors ATP turnover and is easy to implement, requires no additional reagents and can potentially be applied to GTP. We validated the method's accuracy, applied it to the monitoring of ATP turnover by actin and to the screening of ATPase inhibitors, and showed that it is also applicable for the monitoring of GTP hydrolysis.

