[Determination of nitroglycerin in human plasma using gas chromatography after the administration of dosage forms...

An analytical method was developed to determine nitroglycerin in human plasma. The analytical method is based on the extraction of plasma with hexane and the feeding of the packed collumn (10% OV 101) of the gas chromatographic apparatus with the concentrated hexane phase. After separation, detection is carried out by a detector of electron capture. The described method is sensitive (limit of detection = 50 pg/ml of plasma) and sufficiently precise (error of the method = 13%). The precision of the method during the day (+/- 12%) and between the days (+/- 8%) is presented, as well as the return of nitroglycerin from plasma (95%). An application of the method on comparing the pharmacokinetic parameters in two dosage forms used in Czechoslovakia is shown. The results after the administration of one mg of the sublingual and spray forms of nitroglycerin preparations are documented by the course of the levels in the individual experimental subjects and the principal pharmacokinetic parameters derived from the one-compartmental model.