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Updated: Apr 24, 2026
Metabolic Pathway Confirmation and Discovery Through 13C-labeling of Proteinogenic Amino Acids
Published on: January 26, 2012
GC-MS-based ¹³C metabolic flux analysis
Judith Becker1, Christoph Wittmann
1Institute of Systems Biotechnology, Saarland University, Campus A 1 5, 66123, Saarbrücken, Germany.
Abstract:
The in vivo analysis of metabolic fluxes has become a valuable method for the investigation of microorganisms. It turned out especially useful in industrial biotechnology for the prediction of beneficial genetic targets for rational strain optimization. Here, we describe in detail the procedure for the state-of-the-art approach of (13)C metabolic flux analysis comprising steady-state cultivations in a mineral salt medium with (13)C-labeled substrates, GC-MS measurement for labeling analysis, as well as metabolic modeling using the open-source software OpenFlux.
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