Related Experiment Video
Updated: Apr 24, 2026

Preparation of Polypentafluorophenyl acrylate Functionalized SiO2 Beads for Protein Purification
Published on: November 19, 2018
Facile preparation of polyvinyl alcohol coated SiO2 stationary phases for high performance liquid chromatography
Shunli Ji1, Feifang Zhang, Shengjie Wu
1Pharmacy School, East-China Univ. Sci. Tech., 130 Meilong RD, Shanghai 200237, China. zhangff@ecust.edu.cn bcyang@ecust.edu.cn.
Abstract:
A facile method to prepare a polar stationary phase for hydrophilic interaction chromatography (HILIC) was proposed by coating polyvinyl alcohol onto silica particles (PVA-Sil). A water or organic solvent-insoluble permanent PVA coating on the silica particle surface can be formed simply by dipping silica particles into a hot PVA solution and then settled from this solution, leaving a thin layer of PVA coating and frozen in a freezer. The PVA-Sil shields the silica core from solution erosion to some degree and the pH tolerance range was extended to 9.5 from 8.0 for bare silica. PVA-Sil demonstrated a good hydrophilic property for several kinds of polar compounds and ∼57000 m(-1) of plate count was achieved. This method can also be extended as a universal method to prepare various stationary phases with exchangeable functionalities by doping the desired ingredient in a PVA solution.
More Related Videos
13:36Simple In-House Ultra-High Performance Capillary Column Manufacturing with the FlashPack Approach
Published on: December 4, 2021
10:14Synthesis and Purification of Iodoaziridines Involving Quantitative Selection of the Optimal Stationary Phase for Chromatography
Published on: May 16, 2014
Related Concept Videos
High-Performance Liquid Chromatography: Introduction
In HPLC, two phases play a critical role in the separation process:
Gas Chromatography: Types of Columns and Stationary Phases
For an analyte to remain on the column for a sufficient amount of time, it must exhibit some level of compatibility (or...
Silica Gel Column Chromatography: Overview
Polar components tend to bind strongly to the silica gel, causing them to move slowly through the column. In contrast, nonpolar compounds...
High-Performance Liquid Chromatography: Elution Process
Supercritical Fluid Chromatography
SFC utilizes a supercritical fluid mobile phase,...
Ion Exchange