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Updated: Apr 24, 2026

Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions
Published on: September 25, 2018
Detection of multiple mutations in a single codon of genomic DNA
Danishmalik Rafiq Sayyed1, Satish Balasaheb Nimse, Keum-Soo Song
1Institute for Applied Chemistry and Department of Chemistry, Hallym University, Chuncheon, 200-702, Korea. tskim@hallym.ac.kr.
Abstract:
The controller DNA technology allows the detection of multiple mutations in a single codon of genomic DNA. In this technology, the controller DNA is used to control the hybridization of target DNAs with the immobilized DNAs. The controller DNA technology is rapid, specific, and cost-effective for the following reasons, (i) final results in 40 min after PCR, (ii) detection and discrimination of the six mutations in a single codon, and (iii) high sensitivity.

