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Updated: Apr 24, 2026

Structure and Coordination Determination of Peptide-metal Complexes Using 1D and 2D 1H NMR
Published on: December 16, 2013
Disentangling scalar coupling patterns by real-time SERF NMR
Nina Gubensäk1, Walter M F Fabian, Klaus Zangger
1Institute of Chemistry, University of Graz, Heinrichstrasse 28, 8010 Graz, Austria. klaus.zangger@uni-graz.at.
Abstract:
Scalar coupling constants and signal splitting patterns in NMR spectra contain a wealth of short-range structural information. The extraction of these parameters from (1)H NMR spectra is often prohibited by simultaneous scalar coupling interactions with several other protons. Here we present a high-resolution NMR experiment where scalar coupling to only one selected signal is visible. All other couplings are removed from the spectrum. This real-time selectively refocused NMR experiment is achieved by spatially selective homonuclear broadband decoupling combined with selective refocusing during acquisition. It allows the unperturbed extraction of scalar coupling constants from the highly resolved acquisition dimension of NMR spectra.
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