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Published on: July 14, 2023
Bone substitutes enhance osteogenic differentiation of mesenchymal stem cells in three-dimensional scaffolds
Henning Hanken1, Janna Alpers2, Melanie Böbel3
1Department of Oral and Maxillofacial Surgery, University Medical Center Hamburg-Eppendorf, Hamburg, Germany h.hanken@uke.de.
Aim:
The present study aimed to find bone substitutes to enhance osteogenic differentiation of mesenchymal stem cells in three-dimensional scaffolds in the absence of dexamethasone.
Materials And Methods:
Seven commercial bone substitutes were added to a three-dimensional fibrin-matrix containing rat mesenchymal stem cells in a biocompatible poly-L-lactic-acid mesh. Cell viability, cytotoxicity and alkaline phosphatase activity were followed for three weeks. Expression of bone markers was examined by qualitative evaluation of corresponding transcripts.
Results:
Six out of the seven bone derivatives exhibited an osteogenic-enhancing effect.
Conclusion:
The osteogenic-enhancing effect of the evaluated bone substitutes suggests their potential clinical application for preparation of autologous bone replacement material in three-dimensional carriers.
