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Updated: Apr 24, 2026
Colony Formation Assay Detecting the Proliferative Capacity of LncRNA-knockdown Osteosarcoma Cells
Published on: January 16, 2026
Nuclear ING2 expression is reduced in osteosarcoma
Xiao-Rui Han1, Xi-Zhuang Bai1, Yu Sun1
1Department of Joint Surgery and Sports Medicine, The First Affiliated Hospital of China Medical University, Shenyang, Liaoning 110001, P.R. China.
Abstract:
Osteosarcoma is a high-grade malignant bone tumor. Loss of inhibitor of growth 2 (ING2) expression has been demonstrated in numerous types of cancers. However, no study has shown the relationship between ING2 expression and osteosarcoma. In the present study, we confirmed that the levels of ING2 mRNA and protein were lower in cancer tissues than these levels in normal tissues. Loss of nuclear ING2 protein was significantly associated with a decreased survival time of patients. Osteosarcoma cells were transfected with ING2 protein without a nuclear localization signal or intact ING2 protein to examine the effects of exogenous expression of ING2 in vitro. Compared to the control cells, intact ING2-expressing cells exhibited increased apoptosis, G1 phase arrest and senescence. Taken together, these results suggest that ING2 acts as a tumor suppressor in osteosarcoma.
Insights
Inhibitor of growth 2 (ING2) is downregulated in osteosarcoma, correlating with poorer patient survival. Restoring ING2 suppresses tumor growth by increasing apoptosis and cell cycle arrest, suggesting ING2 is a tumor suppressor.
Area of Science:
- Oncology
- Molecular Biology
- Cancer Research
Background:
- Osteosarcoma is a prevalent and aggressive bone cancer.
- Reduced expression of inhibitor of growth 2 (ING2) is observed in various cancers.
- The role of ING2 in osteosarcoma remains unexplored.
Purpose of the Study:
- To investigate the expression levels of ING2 in osteosarcoma.
- To determine the correlation between ING2 expression and patient prognosis.
- To elucidate the functional role of ING2 in osteosarcoma progression.
Main Methods:
- Quantitative analysis of ING2 mRNA and protein in osteosarcoma tissues versus normal tissues.
- Correlation analysis of nuclear ING2 protein levels with patient survival data.
- In vitro studies involving transfection of osteosarcoma cells with ING2 variants to assess cellular effects.
Main Results:
- ING2 mRNA and protein levels were significantly lower in osteosarcoma tissues compared to normal tissues.
- Loss of nuclear ING2 expression was associated with decreased patient survival.
- Exogenous expression of intact ING2 in osteosarcoma cells led to increased apoptosis, G1 phase arrest, and senescence.
Conclusions:
- ING2 functions as a tumor suppressor in osteosarcoma.
- Downregulation of ING2 is a potential biomarker for poor prognosis in osteosarcoma patients.
- Targeting ING2 may offer a therapeutic strategy for osteosarcoma.
