A sentinel protein assay for simultaneously quantifying cellular processes
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Martin Soste1, Rita Hrabakova2, Stefanie Wanka3
1Institute of Biochemistry, Department of Biology, ETH Zurich, Zurich, Switzerland.
Nature Methods
|September 8, 2014
Abstract:
We describe a proteomic screening approach based on the concept of sentinel proteins, biological markers whose change in abundance characterizes the activation state of a given cellular process. Our sentinel assay simultaneously probed 188 biological processes in Saccharomyces cerevisiae exposed to a set of environmental perturbations. The approach can be applied to analyze responses to large sets of uncharacterized perturbations in high throughput.

