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Updated: Apr 24, 2026

Using High Content Imaging to Quantify Target Engagement in Adherent Cells

Using High Content Imaging to Quantify Target Engagement in Adherent Cells

Published on: November 29, 2018

A sentinel protein assay for simultaneously quantifying cellular processes.

Martin Soste¹, Rita Hrabakova², Stefanie Wanka³

¹Institute of Biochemistry, Department of Biology, ETH Zurich, Zurich, Switzerland.

|September 8, 2014

Summary

This summary is machine-generated.

Researchers developed a proteomic screening method using sentinel proteins to monitor 188 cellular processes in yeast. This high-throughput approach efficiently analyzes responses to environmental changes.

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Published on: July 31, 2011

Area of Science:

Proteomics
Systems Biology
Yeast Genetics

Background:

Understanding cellular responses to environmental stimuli is crucial in biology.
Proteomics offers a powerful lens to study these complex cellular dynamics.
Sentinel proteins serve as indicators for specific cellular process activation.

Purpose of the Study:

To introduce a novel proteomic screening approach utilizing sentinel proteins.
To simultaneously analyze a broad range of biological processes.
To enable high-throughput analysis of cellular responses to perturbations.

Main Methods:

Development of a sentinel protein-based assay.
Simultaneous probing of 188 biological processes.
Application to Saccharomyces cerevisiae under various environmental perturbations.

Main Results:

Demonstration of a functional proteomic screening approach.
Successful simultaneous monitoring of 188 biological processes.
Validation of the assay's utility in analyzing environmental responses.

Conclusions:

The sentinel protein assay provides an efficient method for high-throughput proteomic screening.
This approach facilitates the analysis of cellular responses to numerous uncharacterized perturbations.
The methodology is applicable to diverse biological systems and research questions.