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Updated: Apr 24, 2026
Radiosensitivity of Cancer Stem Cells in Lung Cancer Cell Lines
Published on: August 21, 2019
Microarray analysis of gene expression in lung cancer cell lines treated by fractionated irradiation
Sung-Ja Ahn1, Chan Choi2, Yoo-Duk Choi3
1Department of Radiation Oncology, Chonnam National University Medical School, Gwangju, Republic of Korea.
Background:
To identify differentially expressed genes between parent and radioresistant lung cancer cell lines established by fractionated irradiation.
Materials And Methods:
Lung cancer cell lines (A549, NCI-H1650) were irradiated with several fractionation schemes. Clonogenic assays were used to identify radioresistant cell lines. We compared the gene expression profiles on a cDNA microarray.
Results:
Four established cell (A549-2G, A549-5G, H1650-2G and H1650-5G) were shown to be radioresistant (p≤0.05). Seventy-two genes were commonly altered in A549-G and 655 genes in H1650-G, compared to their parental cells. Genes in the wingless-type MMTV integration site family (WNT) signaling pathway were the ones most frequently altered in both A549-G and H1650-G cells. Those involved in inflammation; integrin, platelet-derived growth factor (PDGF), interleukin, transforming growth factor-beta (TGFB), epidermal growth factor receptor (EGFR) signaling, were commonly altered in radioresistant H1650 sublines.
Conclusion:
The major gene expression changes during irradiation are related to WNT signaling pathway.
