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Updated: Apr 24, 2026
Development and Testing of Species-specific Quantitative PCR Assays for Environmental DNA Applications
Published on: November 5, 2020
Development and evaluation of microsatellite markers for a native prairie perennial, Echinacea angustifolia
Jennifer L Ison1, Stuart Wagenius2, Diedre Reitz3
1Department of Biological Sciences, University of Illinois at Chicago, 845 West Taylor Street, Chicago, Illinois 60607 USA ; Plant Conservation and Biology, Chicago Botanic Garden, 1000 Lake Cook Road, Glencoe, Illinois 60022 USA.
Premise Of The Study:
Microsatellite loci for the native prairie perennial Echinacea angustifolia were developed and evaluated for future use in population structure and paternity studies. •
Methods And Results:
A total of 50 trinucleotide microsatellite regions were identified though an enrichment protocol that prescreens for microsatellite repeats before ligating into a vector. Of these, 11 loci were polymorphic and in Hardy-Weinberg equilibrium in three populations with varying numbers of plants. The loci had between three and 14 alleles and collectively provided high paternity exclusion probabilities. •
Conclusions:
These sets of microsatellite primers will provide researchers and land managers with valuable information on the population genetic structure and gene flow between fragmented prairie populations.
