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CRISPR

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...

CRISPR

CRISPR

CRISPR/Cas9 Genome Editing

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...

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Related Experiment Video

Updated: Apr 24, 2026

Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9

Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9

Published on: January 3, 2015

Precise gene deletion and replacement using the CRISPR/Cas9 system in human cells.

Qiupeng Zheng¹, Xiaohong Cai², Meng How Tan³

¹Fudan University Shanghai Cancer Center, Institute of Biomedical Sciences, and Department of Oncology, Shanghai Medical School, Fudan University, Shanghai, China.

|September 12, 2014

Summary

The CRISPR/Cas9 gene editing tool precisely deletes or replaces genes in human cells using two guide RNAs. This technology enables efficient genome modification for studying gene functions.

Keywords:

CRISPR/Cas9 Genome editing deletion replacement

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Last Updated: Apr 24, 2026

Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9

Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9

Published on: January 3, 2015

Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells

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Published on: September 25, 2019

CRISPR-Cas9 Mediated Gene Deletion in Human Pluripotent Stem Cells Cultured Under Feeder-Free Conditions

CRISPR-Cas9 Mediated Gene Deletion in Human Pluripotent Stem Cells Cultured Under Feeder-Free Conditions

Published on: November 1, 2024

Area of Science:

Molecular Biology
Genetics
Biotechnology

Background:

The prokaryotic CRISPR/Cas9 system is a powerful tool for genome editing.
CRISPR/Cas9 has been adapted for use in various cells and model organisms.

Purpose of the Study:

To describe targeted gene deletion and replacement in human cells using the CRISPR/Cas9 system with two guide RNAs.
To evaluate the efficiency and precision of CRISPR/Cas9-mediated genome editing.

Main Methods:

Utilized the CRISPR/Cas9 system with two guide RNAs for targeted gene manipulation in human cells.
Employed a homology repair donor template for precise gene replacement experiments.

Main Results:

Achieved efficient targeted gene deletions of various lengths, irrespective of the target gene's transcriptional status.
Demonstrated high efficiency in forming correct junctions for targeted gene deletions.
Successfully guided precise gene replacement when a homology repair donor was provided.

Conclusions:

The CRISPR/Cas9 system effectively and precisely generates targeted gene deletions and replacements in human cells.
This methodology facilitates the characterization of functional domains in protein-coding genes and noncoding regulatory sequences.
CRISPR/Cas9 is a valuable tool for advancing genomic research in human and animal systems.