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Updated: Apr 24, 2026

Phase Behavior of Charged Vesicles Under Symmetric and Asymmetric Solution Conditions Monitored with Fluorescence Microscopy
Published on: October 24, 2017
Human serotonin receptor 5-HT(1A) preferentially segregates to the liquid disordered phase in synthetic lipid
M Gertrude Gutierrez1, Noah Malmstadt
1Mork Family Department of Chemical Engineering and Materials Science, University of Southern California , 925 Bloom Walk, Los Angeles, California 90089, United States.
Abstract:
We demonstrate successful incorporation of the G protein coupled receptor 5-HT1A into giant unilamellar vesicles using an agarose rehydration method. With direct observation using fluorescence techniques, we report preferential segregation of 5-HT1A into the cholesterol-poor liquid disordered phase of the membrane, contradicting previous reports of lipid raft segregation. Furthermore, altering the concentration of cholesterol and sphingomyelin in ternary mixtures does not alter 5-HT1A segregation into the liquid disordered phase.
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