Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

In vitro Mutagenesis01:16

In vitro Mutagenesis

4.7K
4.7K
In-vitro Mutagenesis01:16

In-vitro Mutagenesis

14.8K
To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
14.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Purification of Human Cytoplasmic Actins From <i>Saccharomyces cerevisiae</i>.

Bio-protocol·2023
Same author

Purification of human β- and γ-actin from budding yeast.

Journal of cell science·2023
Same author

Biochemical characterization of actin assembly mechanisms with ALS-associated profilin variants.

European journal of cell biology·2022
Same author

Replication stress induces global chromosome breakage in the fragile X genome.

Cell reports·2021
Same author

Replication Stress Induces Global Chromosome Breakage in the Fragile X Genome.

Cell reports·2020
Same author

Classical Genetics with Saccharomyces cerevisiae.

Cold Spring Harbor protocols·2016
Same journal

Nanotechnology-Stem Cell Strategies in 3D Glioblastoma Organoid: Targeting Glioma Stem Cells Within a Complex Tumor Microenvironment.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Mapping the 3D Chromosome Organization of a Biosynthetic Gene Cluster by Capture Hi-C (CHi-C).

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Mapping the 3D Chromosome Organization of Streptomyces by Hi-C.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

CUT&Tag Epigenomic Profiling of Biosynthetic Gene Clusters in Arabidopsis thaliana.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Rhizobium rhizogenes-Mediated Hairy Root Transformation Protocol for Lotus japonicus and Other Legumes.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Characterization of Bioactive Saponins from Sea Cucumbers.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Related Experiment Video

Updated: Apr 24, 2026

Author Spotlight: Enhanced Isolation of Interaction-Null Mutants in Yeast
02:44

Author Spotlight: Enhanced Isolation of Interaction-Null Mutants in Yeast

Published on: December 29, 2023

1.1K

Using two-hybrid interactions to identify separation-of-function mutations.

Brian Haarer1, David C Amberg

  • 1Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, 750 E. Adams St., Syracuse, NY, 13210, USA.

Methods in Molecular Biology (Clifton, N.J.)
|September 13, 2014
PubMed
Summary
This summary is machine-generated.

Identifying protein interactions is crucial for understanding cellular processes. This study presents a simple yeast two-hybrid method to find specific separation-of-function mutations, which are vital for dissecting protein roles.

More Related Videos

Unravelling the Function of a Bacterial Effector from a Non-cultivable Plant Pathogen Using a Yeast Two-hybrid Screen
11:30

Unravelling the Function of a Bacterial Effector from a Non-cultivable Plant Pathogen Using a Yeast Two-hybrid Screen

Published on: January 20, 2017

11.5K
Split-Ubiquitin Based Membrane Yeast Two-Hybrid MYTH System: A Powerful Tool For Identifying Protein-Protein Interactions
14:04

Split-Ubiquitin Based Membrane Yeast Two-Hybrid MYTH System: A Powerful Tool For Identifying Protein-Protein Interactions

Published on: February 1, 2010

30.7K

Related Experiment Videos

Last Updated: Apr 24, 2026

Author Spotlight: Enhanced Isolation of Interaction-Null Mutants in Yeast
02:44

Author Spotlight: Enhanced Isolation of Interaction-Null Mutants in Yeast

Published on: December 29, 2023

1.1K
Unravelling the Function of a Bacterial Effector from a Non-cultivable Plant Pathogen Using a Yeast Two-hybrid Screen
11:30

Unravelling the Function of a Bacterial Effector from a Non-cultivable Plant Pathogen Using a Yeast Two-hybrid Screen

Published on: January 20, 2017

11.5K
Split-Ubiquitin Based Membrane Yeast Two-Hybrid MYTH System: A Powerful Tool For Identifying Protein-Protein Interactions
14:04

Split-Ubiquitin Based Membrane Yeast Two-Hybrid MYTH System: A Powerful Tool For Identifying Protein-Protein Interactions

Published on: February 1, 2010

30.7K

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Cellular functions rely on protein-protein interactions to regulate biochemical processes.
  • Traditional genetic analysis, using null or temperature-sensitive alleles, can be limited in dissecting specific protein interaction contributions.
  • Understanding how individual interactions contribute to overall protein function requires specialized mutations.

Purpose of the Study:

  • To describe a straightforward protocol for identifying separation-of-function mutations.
  • To leverage the yeast two-hybrid system for discovering mutations that selectively disrupt specific protein interactions.
  • To provide a method for researchers to dissect the relative importance of different protein-protein interactions.

Main Methods:

  • Utilizing the yeast two-hybrid system, a well-established technique for detecting protein-protein interactions.
  • Developing a protocol accessible to laboratories with standard molecular biology expertise.
  • Employing mutational analysis in conjunction with the yeast two-hybrid system.

Main Results:

  • A simple and accessible protocol for identifying separation-of-function mutations using the yeast two-hybrid system has been established.
  • The method allows for the targeted isolation of mutations that affect specific protein-protein interactions without abolishing all protein function.
  • This approach facilitates the dissection of complex protein interaction networks.

Conclusions:

  • The described yeast two-hybrid based protocol offers a valuable tool for geneticists and molecular biologists.
  • It enables the precise study of protein interaction specificity and its role in cellular functions.
  • This method overcomes limitations of traditional genetic approaches for dissecting protein interaction networks.