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Cyclooxygenase inhibition does not alter methacholine-induced sweating.
Naoto Fujii1, Ryan McGinn1, Gabrielle Paull1
1Human and Environmental Physiology Research Unit, School of Human Kinetics, University of Ottawa, Ottawa, Ontario, Canada.
Journal of Applied Physiology (Bethesda, Md. : 1985)
|September 13, 2014
Summary
This study investigated the roles of nitric oxide (NO) and cyclooxygenase (COX) in cholinergic-induced sweating and vasodilation. Findings indicate NO synthase and COX inhibition do not affect methacholine-induced sweating.
Area of Science:
- Physiology
- Dermatology
- Pharmacology
Background:
- Cholinergic agents stimulate cutaneous vasodilation and sweating.
- Nitric oxide (NO) and cyclooxygenase (COX) are implicated in cholinergic vasodilation.
- The role of COX in cholinergic sweating remains unclear.
Purpose of the Study:
- To determine the involvement of NO and COX in methacholine-induced cutaneous vasodilation and sweating.
- To elucidate the specific pathways mediating these physiological responses.
Main Methods:
- Healthy adults underwent intradermal microdialysis with lactated Ringer, ketorolac (COX inhibitor), l-NAME (NO synthase inhibitor), or combined ketorolac + l-NAME.
- Methacholine was infused in a dose-dependent manner to assess forearm sweat rate and cutaneous vascular conductance (CVC).
Main Results:
- Ketorolac alone did not affect CVC.
- l-NAME and combined ketorolac + l-NAME significantly reduced CVC at higher methacholine doses.
- Neither NO synthase nor COX inhibition altered methacholine-induced forearm sweating.
Conclusions:
- Nitric oxide synthase, not cyclooxygenase, mediates cholinergic cutaneous vasodilation.
- Cholinergic sweating is independent of both nitric oxide and cyclooxygenase pathways.
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