Related Experiment Video
Updated: Apr 24, 2026

A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products
Published on: October 4, 2019
Preparative separation and purification of gingerols from ginger (Zingiber officinale Roscoe) by high-speed
Kunyou Zhan1, Kun Xu2, Hongzong Yin3
1Office of Academic Affairs, Shandong Agricultural University, Tai'an 271018, China; State Key Laboratory of Crop Biology, Tai'an 271018, Shandong, China.
Abstract:
A novel method for purifying gingerols from ginger was developed using a high-speed counter-current chromatography (HSCCC). The two-phase solvent system such as light petroleum (bp 60-90°C)-ethyl acetate-methanol-water (5:5:6.5:3.5, v/v/v/v) was applied to the separation and purification of 6-, 8- and 10-gingerol from a crude extract of ginger. The experiment yielded 30.2mg of 6-gingerol, 40.5mg of 8-gingerol, 50.5mg of 10-gingerol from 200mg of crude extract in one-step separation. And the purity of these compounds was 99.9%, 99.9% and 99.2%, respectively, as determined by high-performance liquid chromatography (HPLC). Their structures were identified by gas chromatography-mass spectrometry (GC/MS) and (1)H, (13)C nuclear magnetic resonance (NMR).
Related Concept Videos
Gas Chromatography: Introduction
In GC, a sample is vaporized and mixed with an inert carrier gas (the mobile phase), which transports it through a...
Principles Of Column Chromatography
High-Performance Liquid Chromatography: Introduction
In HPLC, two phases play a critical role in the separation process:
Supercritical Fluid Chromatography
SFC utilizes a supercritical fluid mobile phase,...
Gas Chromatography–Mass Spectrometry (GC–MS)
A gas chromatograph consists of a long, narrow capillary column with a polysiloxane coating on the inner wall....
Optimizing Chromatographic Separations
Band broadening refers to spreading solute bands as they travel through the column. This broadening can impact resolution. Plate height (H) represents the length required for one theoretical plate. A lower plate height corresponds to...

