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Updated: Apr 24, 2026

Qualitative and Quantitative Assays for Detection and Characterization of Protein Antimicrobials
Published on: April 10, 2016
Prevalence and stability of lysozyme in cheese
N Schneider1, K Werkmeister2, C-M Becker3
1Department of Chemistry and Pharmacy, Food Chemistry, Emil-Fischer-Center, University of Erlangen-Nuremberg, Schuhstr. 19, 91052 Erlangen, Germany.
Abstract:
The use of the preservative and potential allergen hen egg white lysozyme in cheese production has to be declared. In the present study, an HPLC method with fluorescence detection (HPLC-FLD) was optimised and validated for the analysis of lysozyme in cheese. Lysozyme was detected in concentrations between 30.8 and 386.2mg/kg cheese in 30 out of 46 analysed commercial cheese samples. During cheese production and storage for 0-54weeks a lysozyme satellite peak (LSP) was detected, which totals up to 18% of the lysozyme content. Mass spectrometry and peptide mass fingerprint revealed that LSP possesses the same primary structure as lysozyme. Since disulphide scrambling could not be detected, LSP was assigned to a conformational isomer of lysozyme. As a consequence, LSP was included in the HPLC-FLD analysis of lysozymes in cheese.
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