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Related Experiment Video

Updated: Apr 24, 2026

A Laser-induced Mouse Model of Chronic Ocular Hypertension to Characterize Visual Defects
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A laser-induced mouse model with long-term intraocular pressure elevation.

Hongmin Yun1, Kira L Lathrop2, Enzhi Yang1

  • 1Department of Ophthalmology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, United States of America; Louis J. Fox Center for Vision Restoration, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, United States of America.

Plos One
|September 13, 2014
PubMed

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Summary

A new mouse model for glaucoma was created using laser treatment to raise intraocular pressure (IOP). This model shows optic nerve damage and may help test stem cell therapies for glaucoma.

Area of Science:

  • Ophthalmology
  • Regenerative Medicine
  • Animal Models

Background:

  • Glaucoma is a leading cause of irreversible blindness.
  • Current treatments aim to lower intraocular pressure (IOP) but do not restore damaged optic nerves.
  • Novel therapeutic strategies, including stem cell-based approaches, are needed for glaucoma treatment.

Purpose of the Study:

  • To develop and characterize a mouse model with elevated intraocular pressure (IOP) induced by laser photocoagulation of the trabecular meshwork (TM).
  • To establish a preclinical model for investigating the efficacy of stem cell-based therapies in glaucoma.

Main Methods:

  • Intraocular pressure (IOP) was measured in C57BL/6 mice.
  • Unilateral laser photocoagulation of the TM was performed to induce IOP elevation.

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  • Optic nerve damage was assessed using electroretinography and axon counting.
  • TM changes were evaluated via histology, immunofluorescence, OCT, and TEM.

    • Main Results:

    • Laser treatment successfully induced sustained IOP elevation (>20 mmHg) in 57% of eyes for 24 weeks.
    • Significant optic nerve axon loss (59%) and decreased Photopic Negative Response (PhNR) were observed.
    • Histology and OCT confirmed an open anterior chamber angle without synechiae.
    • TEM revealed TM extracellular matrix disorganization and inflammatory cell infiltration.

    Conclusions:

    • Laser photocoagulation effectively created a murine model of open-angle ocular hypertension with optic nerve damage.
    • This model serves as a valuable tool for evaluating stem cell therapies aimed at restoring TM function and integrity in glaucoma.