Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Transcription Factors02:16

Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
Position-effect Variegation02:32

Position-effect Variegation

In 1928, a German botanist Emil Heitz observed the moss nuclei with a DNA binding dye. He observed that while some chromatin regions decondense and spread out in the interphase nucleus, others do not. He termed them euchromatin and heterochromatin, respectively. He proposed that the heterochromatin regions reflect a functionally inactive state of the genome. It was later confirmed that heterochromatin is transcriptionally repressed, and euchromatin is transcriptionally active chromatin.
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
Multi-species Conserved Sequences02:51

Multi-species Conserved Sequences

Next-generation sequencing technologies have created large genomic databases of a variety of animals and plants. Ever since the human genome project was completed, scientists studied the genome of primates, mammals, and other phylogenetically distant living beings. Such large-scale  studies have provided new insights into the evolutionary relationship between organisms.
Although the genome of each species varies greatly from each other, a few sequences are highly conserved. Such conserved DNA...
General Transcription Factors01:30

General Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Physicians and artificial intelligence diverge in evaluating large language models on real clinical cases.

NPJ digital medicine·2026
Same author

Intracranial Pressure Reduction Is Associated with Mitochondrial OPA1 and Cytochrome c Release in the Retinas of AQP1-Null Mice.

Brain sciences·2026
Same author

Optic nerve intraneural pressure trend under intraocular-intracranial pressure effects.

The British journal of ophthalmology·2026
Same author

Bioengineered 3D Human Trabecular Meshwork Models for Outflow Physiology and Glaucoma Research.

Bioengineering (Basel, Switzerland)·2026
Same author

NRF2 Deficiency Disrupts Mitochondrial Homeostasis via NDUFS7 in Trabecular Meshwork.

Research (Washington, D.C.)·2026
Same author

Retinal vascular alteration following surgical intraocular pressure reduction in primary angle closure disease.

BMJ open ophthalmology·2026
Same journal

Correction: Wang et al. Phosphatidylserine Decarboxylase Promotes Ferroptosis Through STAT3/GPX4 Signaling in Gastric Cancer. <i>Curr. Issues Mol. Biol.</i> 2026, <i>48</i>, 300.

Current issues in molecular biology·2026
Same journal

Exploring the Relationship Between Protein-Level Ratios (rQLTs) and Duodenal Ulcer.

Current issues in molecular biology·2026
Same journal

Metformin as an Innate Immune Modulator: Metabolic and Epigenetic Reprogramming of Innate Immune Cells and Therapeutic Implications.

Current issues in molecular biology·2026
Same journal

Comprehensive Bioinformatic Characterization of CD70, CD80, and TIGIT as Diagnostic, Prognostic, and Immune Biomarkers in Pan-Cancer.

Current issues in molecular biology·2026
Same journal

Genome-Wide Identification and Expression Analysis of the Thaumatin-like Protein Genes in <i>Filipendula ulmaria</i> under <i>Bipolaris sorokiniana</i> Infection.

Current issues in molecular biology·2026
Same journal

Recent Dominant Transposition Events Affect Gene Regulatory Regions, but Not Coding Sequences, in Polar and Brown Bear Genomes.

Current issues in molecular biology·2026
See all related articles

Related Experiment Video

Updated: May 7, 2026

Isolation and Enrichment of Human Adipose-derived Stromal Cells for Enhanced Osteogenesis
11:00

Isolation and Enrichment of Human Adipose-derived Stromal Cells for Enhanced Osteogenesis

Published on: January 12, 2015

11.7K

Transcriptomic Differences Between Human Trabecular Meshwork Stem Cells and Trabecular Meshwork Cells Reveal Specific

Rong Du1,2, Ajay Kumar3, Enzhi Yang2

  • 1Beijing Institute of Ophthalmology, Beijing Tongren Eye Center, Beijing Tongren Hospital, Beijing Key Laboratory of Intelligent Diagnosis Technology and Equipment for Optic Nerve-Related Eye Diseases, Capital Medical University, Beijing 100730, China.

Current Issues in Molecular Biology
|July 29, 2025
PubMed
Summary
This summary is machine-generated.

Trabecular meshwork stem cells (TMSCs) and trabecular meshwork cells (TMCs) have distinct molecular profiles. Identifying these differences provides key biomarkers for developing novel glaucoma stem cell therapies.

Keywords:
RNA sequencingglaucomahub genesmicroarraystem cellstrabecular meshworktranscriptomic profiling

More Related Videos

In Vitro Differentiation of Human Mesenchymal Stem Cells into Functional Cardiomyocyte-like Cells
09:05

In Vitro Differentiation of Human Mesenchymal Stem Cells into Functional Cardiomyocyte-like Cells

Published on: August 9, 2017

11.0K
Laser Capture Microdissection of Highly Pure Trabecular Meshwork from Mouse Eyes for Gene Expression Analysis
13:47

Laser Capture Microdissection of Highly Pure Trabecular Meshwork from Mouse Eyes for Gene Expression Analysis

Published on: June 3, 2018

9.4K

Related Experiment Videos

Last Updated: May 7, 2026

Isolation and Enrichment of Human Adipose-derived Stromal Cells for Enhanced Osteogenesis
11:00

Isolation and Enrichment of Human Adipose-derived Stromal Cells for Enhanced Osteogenesis

Published on: January 12, 2015

11.7K
In Vitro Differentiation of Human Mesenchymal Stem Cells into Functional Cardiomyocyte-like Cells
09:05

In Vitro Differentiation of Human Mesenchymal Stem Cells into Functional Cardiomyocyte-like Cells

Published on: August 9, 2017

11.0K
Laser Capture Microdissection of Highly Pure Trabecular Meshwork from Mouse Eyes for Gene Expression Analysis
13:47

Laser Capture Microdissection of Highly Pure Trabecular Meshwork from Mouse Eyes for Gene Expression Analysis

Published on: June 3, 2018

9.4K

Area of Science:

  • Ophthalmology
  • Regenerative Medicine
  • Genomics

Background:

  • Glaucoma is a leading cause of irreversible blindness.
  • Trabecular meshwork (TM) dysfunction is a primary cause of glaucoma.
  • Trabecular meshwork stem cells (TMSCs) show potential for TM regeneration therapies, but their molecular identity is unclear.

Purpose of the Study:

  • To comprehensively compare the molecular characteristics of human TMSCs and human TM cells (TMCs).
  • To identify distinct molecular signatures and potential biomarkers for TMSCs and TMCs.
  • To lay the groundwork for future stem cell-based glaucoma therapies.

Main Methods:

  • RNA sequencing and microarray analyses were used for transcriptomic comparison.
  • Quantitative PCR (qPCR) was employed for validation of gene expression.
  • Functional enrichment and network topology analyses were performed to interpret gene expression data.

Main Results:

  • A total of 465 differentially expressed genes were identified between TMSCs and TMCs.
  • TMSCs showed enrichment in development, immune signaling, and extracellular matrix remodeling pathways.
  • TMCs were enriched in structural, contractile, and adhesion-related functions, with specific hub genes identified.

Conclusions:

  • Distinct molecular signatures differentiate TMSCs and TMCs.
  • Identified genes like CXCL3, CXCL6, BMP2, LMOD1, and BGN serve as reliable biomarkers for cell identity.
  • These findings provide a foundation for developing targeted stem cell therapies for glaucoma.