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Published on: November 23, 2011
In situ hybridization freeze-assisted punches (IFAP): technique for liquid-based tissue extraction from thin
Lawrence S Own1, Paresh D Patel
1Molecular and Behavioral Neuroscience Institute, University of Michigan, 205 Zina Pitcher Place, Biomedical Science Research Building, Room 5698, Ann Arbor, MI, 48109, USA, lsown@voxa.co.
Abstract:
In situ hybridization-assisted punches (IFAP) are a low-cost method for extracting tissue from frozen slide-mounted sections as thin as 12 μm. The method synergizes well with standard histological workflows and uses in situ hybridization to target corresponding slide-mounted cryosections that contain the region of interest. Liquid beads of M-1 embedding matrix are applied and snap frozen, binding the matrix to the underlying tissue. Bead-tissue complexes are removed and DNA extracted using a high-salt method. IFAP-extracted DNA is suitable for downstream DNA methylation analysis.
