Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

1.9K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
1.9K
Yeast Signaling01:28

Yeast Signaling

15.6K
Yeasts are single-celled organisms, but unlike bacteria, they are eukaryotes (cells with a nucleus). Cell signaling in yeast is similar to signaling in other eukaryotic cells. A ligand, such as a protein or a small molecule released from a yeast cell, attaches to a receptor on the cell surface. The binding stimulates second-messenger kinases to activate or inactivate transcription factors that further regulate gene expression. Many of the yeast intracellular signaling cascades have similar...
15.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Barbaloin Alleviates Lung Ischemia-Reperfusion Injury by Dual-Targeting IL-6 and PNP.

International journal of molecular sciences·2026
Same author

Mapping and engineering the human cell-cell interactome.

Nature biotechnology·2026
Same author

Programmable pathway profiles reveal signaling principles of TGF-β superfamily receptors.

bioRxiv : the preprint server for biology·2026
Same author

Development of National Anticoagulation Guidelines for Pregnant Women with Mechanical Heart Valves in Rwanda.

Cardiovascular journal of Africa·2026
Same author

Staged Hybrid Treatment of a Large Aneurysmal Pulmonary Sequestration With Thoracic Endovascular Aortic Repair Followed by Lobectomy.

Annals of thoracic surgery short reports·2026
Same author

Persistent Benefits of Early Gestational Chenodeoxycholic Acid Supplementation on Late Pregnancy in Sows Via Sustained Modulation of the Gut-Metabolism Axis.

The Journal of nutrition·2026
Same journal

Pitch selectivity in ferret auditory cortex.

Current biology : CB·2026
Same journal

A cell size-dependent competition between geometry and polarity governs nuclear and spindle positioning in early embryos.

Current biology : CB·2026
Same journal

Trophic cascades drive sustainability in the agricultural heritage rice-fish coculture system.

Current biology : CB·2026
Same journal

Tracking Satb2-positive retinal ganglion cells in zebrafish unveils developmental functional reorganization.

Current biology : CB·2026
Same journal

RhoGAP54D promotes cell size asymmetry and inhibits pulsatile myosin activity in Drosophila neural stem cells.

Current biology : CB·2026
Same journal

Increased rates of hybridization in swordtails are associated with water pollution.

Current biology : CB·2026
See all related articles

Related Experiment Video

Updated: Apr 23, 2026

Budding Yeast Protein Extraction and Purification for the Study of Function, Interactions, and Post-translational Modifications
09:22

Budding Yeast Protein Extraction and Purification for the Study of Function, Interactions, and Post-translational Modifications

Published on: October 30, 2013

24.9K

Pulsatile dynamics in the yeast proteome.

Chiraj K Dalal1, Long Cai1, Yihan Lin1

  • 1Howard Hughes Medical Institute, Division of Biology and Bioengineering, California Institute of Technology, Pasadena, CA 91125, USA.

Current Biology : CB
|September 16, 2014
PubMed
Summary
This summary is machine-generated.

Cellular regulation involves transcription factors activating in stochastic pulses. A proteome-wide screen in yeast found this pulsing behavior is common among transcription factors, suggesting a pervasive role in cellular dynamics.

More Related Videos

Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast
08:13

Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast

Published on: September 26, 2025

779
Enzymatic Modification and Flow Cytometry Assessment of Yeast Surface Displayed Proteins
10:54

Enzymatic Modification and Flow Cytometry Assessment of Yeast Surface Displayed Proteins

Published on: May 30, 2025

1.0K

Related Experiment Videos

Last Updated: Apr 23, 2026

Budding Yeast Protein Extraction and Purification for the Study of Function, Interactions, and Post-translational Modifications
09:22

Budding Yeast Protein Extraction and Purification for the Study of Function, Interactions, and Post-translational Modifications

Published on: October 30, 2013

24.9K
Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast
08:13

Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast

Published on: September 26, 2025

779
Enzymatic Modification and Flow Cytometry Assessment of Yeast Surface Displayed Proteins
10:54

Enzymatic Modification and Flow Cytometry Assessment of Yeast Surface Displayed Proteins

Published on: May 30, 2025

1.0K

Area of Science:

  • Cellular and Molecular Biology
  • Genetics and Genomics
  • Biophysics

Background:

  • Cellular regulation relies on transcription factor activation, which can occur in stochastic pulses of nuclear localization.
  • The prevalence, variability, and broader role of such pulsing behaviors beyond transcriptional regulators remain unclear.

Purpose of the Study:

  • To systematically identify localization-based pulsing behaviors across the yeast proteome.
  • To determine if pulsing is specific to transcriptional regulators or more widespread.
  • To characterize the dynamics and patterns of observed pulsing behaviors.

Main Methods:

  • Performed a proteome-wide, movie-based screen using a fluorescent protein fusion library in Saccharomyces cerevisiae.
  • Examined 4,159 strains across multiple media conditions to observe protein localization dynamics.
  • Analyzed localization patterns to identify stochastic pulsing events.

Main Results:

  • Identified stochastic pulsing in ten proteins, all of which were transcription factors.
  • Observed heterogeneous and unsynchronized pulse dynamics among cells within clonal populations.
  • Found that pulsing occurs primarily in paralogous and redundant protein pairs.

Conclusions:

  • Pulsatile dynamics are a prevalent behavior among transcription factors in yeast.
  • The observed pulsing is the sole dynamic localization behavior identified in the screen.
  • These findings suggest pulsatile dynamics may play a significant role in eukaryotic cellular regulation.