Related Experiment Video
Updated: Apr 23, 2026

3D Imaging of PDL Collagen Fibers during Orthodontic Tooth Movement in Mandibular Murine Model
Published on: April 15, 2021
Volumetric pulp changes after orthodontic treatment determined by cone-beam computed tomography
Shivanand Venkatesh1, Shreya Ajmera2, Sanjay V Ganeshkar2
1Department of Orthodontics and DentofacialOrthopedics, MS Ramaiah Dental College and Hospital, MSR Nagar, Bangalore, Karnataka, India.
Introduction:
The purpose of this study was to observe and evaluate 3-dimensional pulp cavity changes during orthodontic treatment.
Methods:
Eighty-seven patients formed the study sample and were divided into an experimental group (48 patients) and a control group (39 patients). Cone-beam computed tomographic (CBCT) records were obtained before the start of the treatment (T0) and after space closure for the experimental group, whereas for the control group CBCT images were obtained approximately 17-18 months (T1) after obtaining the first image (T0). CBCT data were reconstructed with surface and volume rendering software (Mimics; Materialise, Leuven, Belgium), and the volumetric images were modified to display the teeth from various orientations. Six anterior teeth were segmented and their pulps isolated. Paired t test was used to check for statistical significance.
Results:
The difference in the pulp volume was statistically significant at P < .05 for all the anterior teeth in the experimental group and at P < .05 for the right canine, P < .05 for the right and left lateral incisors, and P < .05 for the left central in the control group.
Conclusions:
Orthodontic treatment in the experimental group produced a significant decrease in the size of the pulp, which was statistically significant.

