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Updated: Apr 23, 2026
Targeted DNA Methylation Analysis by Next-generation Sequencing
Published on: February 24, 2015
eMethylsorb: electrochemical quantification of DNA methylation at CpG resolution using DNA-gold affinity interactions
Abu Ali Ibn Sina1, Sidney Howell, Laura G Carrascosa
1Australian Institute for Bioengineering and Nanotechnology (AIBN), The University of Queensland, Brisbane, QLD 4072, Australia. m.shiddiky@uq.edu.au lgcarrascosa@uq.edu.au.
Abstract:
We report a simple electrochemical method referred to as "eMethylsorb" for the detection of DNA methylation. The method relies on the base dependent affinity interaction of DNA with gold. The methylation status of DNA is quantified by monitoring the electrochemical current as a function of the relative adsorption level of bisulphite treated DNA samples onto a bare gold electrode. This method can successfully distinguish methylated and unmethylated epigenotypes at single CpG resolution.
