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Updated: Apr 23, 2026
Mouse Models of Periventricular Leukomalacia
Published on: May 18, 2010
[Expression of endogenous leukemia inhibitory factor in neonatal rats with periventricular leukomalacia]
Yu-Ying Fan1, Tao Yu, Jun-Mei Zhang
1Department of Pediatric Neurology, Shengjing Hospital of China Medical University, Shenyang 110004, China. wangh1@sj-hospital.org.
Objective:
To study the changes of endogenous leukemia inhibitory factor (LIF) in neonatal rats with periventricular leukomalacia (PVL).
Methods:
A PVL model of 3-day-old Wistar rats was prepared by left carotid artery ligation followed by 6% oxygen for 4 hours. The rats were sacrificed at 1, 3, 7, 14 and 28 days of hypoxia ischemia (HI), and the brain tissues were sampled. Real-Time PCR and Western blot methods were applied to analyze the expression of LIF mRNA and protein. Double staining immunofluorescence was used to detect the co-expression of LIF and GFAP.
Results:
At 1, 3 and 7 days of HI, LIF protein level in the PVL group was higher than in the control group (P<0.01). In the PVL group, the LIF protein level on the third day after HI reached a peak and was higher than the other time points (P<0.01). The change of LIF mRNA expression showed the same tendency with LIF protein. The double staining immunofluorescence showed a co-expression of LIF and GFAP.
Conclusions:
LIF mRNA and LIF protein expression in astrocytes show a trend of initial increase followed by steady decline in neonatal rats with PVL, suggesting that endogenous LIF may participate in the repair of PVL.
