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Highly potent oligostilbene sbLOX-1 inhibitor from Gnetum macrostachyum
Abstract:
Isolated oligostilbenes from G. macrostachyum exhibited sbLOX-1 inhibitory activity with IC50 values ranging from 0.14-11.91 microM. Of these oligostilbenoids, 12 (IC50 0.14 +/- 0.01 microM), 10 (IC50 0.33 +/- 0.11 microM), 11 (IC50 0.49 +/- 0.05 microM) and 7 (IC50 1.03 +/- 0.43 microM) were more active than nordihydroguaiaretic acid (NDGA) (P < 0.05) which was the positive control. The enzyme kinetic analysis revealed that 7 and 11 inhibited sbLOX-1 noncompetitively with Ki values of 11.2 and 71.4 nM. Compound 10 inhibited sbLOX-1 through mixed-competitive mechanisms (Ki = 13.8 nM and Ki' 56.7 nM). Moreover, 12 was an uncompetitive inhibitor with a Ki value of 0.8 nM. The inhibitory activity of oligostilbenes did not result from the antioxidant property, as demonstrated by DPPH and 13-HPOD scavenging assays. These compounds also showed ferric reducing capabilities, but had no effect in a Fe(3+)-bound sbLOX-1 model, as indicated by UV spectrophotometric and CD spectroscopic studies.
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