Anthranilic acid derivatives and other components from Ononis pusilla

Area of Science:

• Natural Product Chemistry
• Phytochemistry
• Organic Chemistry

Background:

• Ononis pusilla L. is a plant species with potential for yielding bioactive compounds.
• Anthranilic acid derivatives and flavonoids are classes of natural products with diverse biological activities.

Purpose of the Study:

• To isolate and characterize chemical constituents from the ethyl acetate extract of Ononis pusilla L.
• To identify novel compounds and known phytochemicals for further investigation.

Main Methods:

• Extraction of plant material using ethyl acetate.
• Isolation of compounds using chromatographic techniques (implied).
• Structure elucidation through comprehensive spectroscopic methods: 1D and 2D NMR (homo- and heteronuclear), ESI-MS.
• Confirmation of structures by chemical transformation and comparison with existing literature data.

Main Results:

• Isolation of three new anthranilic acid derivatives: N-(R)-3'-hydroxydocosanoylanthranilic acid (1), N-(R)-3'-hydroxytricosanoylanthranilic acid (2), and N-(R)-3'-hydroxytetracosanoylanthranilic acid (3).
• Identification of one known anthranilic acid derivative.
• Isolation of six known flavonoids.

Conclusions:

• The phytochemical investigation of Ononis pusilla L. led to the discovery of novel anthranilic acid derivatives.
• The identified compounds contribute to the known chemical diversity of the Ononis genus.
• Further studies are warranted to explore the potential biological activities of these isolated compounds.