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Updated: Apr 23, 2026

The Cultivation, Growth, and Viability of Lactic Acid Bacteria: A Quality Control Perspective
Published on: June 16, 2022
A microbial biosensor based on Lactobacillus delbruecki sp. bacterial cells for simultaneous determination of lactic
Erhan Canbay1, Alper Habip1, Goknur Kara1
1Department of Biochemistry, Faculty of Science, Ege University, 35100 Bornova-İzmir, Turkey.
Abstract:
The aim of this study was mainly to develop a microbial biosensor for the simultaneous determination of lactic acid and pyruvic acid. In developing biosensor, lyophilised Lactobacillus delbruecki sp. bacterial cells were immobilised with polypyrrole on a platin electrode surface using electropolymerization method. Lactate concentration was determined based on the differences in amperometric responses at cathodic peak (+0.2V) of potassium ferricyanide, whereas pyruvate concentration was determined using the differences at anodic peak (+0.1V). The response of biosensor showed linearity between 0.1 and 1.0mM for both of two substrates. Optimisation studies were carried out for amount of microorganism, pyrrole concentration, pH and temperature. In the characterisation studies, substrate specificity, interference effect of some substances on the biosensor response, and storage stability were established.
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