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Updated: Oct 27, 2026

In vitro Digestion of Emulsions in a Single Droplet via Multi Subphase Exchange of Simulated Gastrointestinal Fluids
Published on: November 18, 2022
Effects of modified β-lactoglobulin on in vitro digestion and absorption characteristics of algae oil emulsion
Rui Wang1, Qingchun Zhang1, Jianfei He1
1College of Food Science and Technology, Zhejiang University of Technology, Hangzhou, 310014, PR China; Research and Experimental Base for Whole Grain Nutrition Food Processing Technology, Ministry of Agriculture and Rural Affairs of the, PR China; State Key Laboratory of Green Chemical Synthesis and Conversion, Zhejiang University of Technology, Hangzhou, 310014, PR China.
Abstract:
In this study, β-lactoglobulin (β-Lg) was modified by sequential glycation, high-pressure microfluidization (HPM), and conjugation with (-)-epigallocatechin-3-gallate (EGCG). The effects of this modified protein on the in vitro digestive and intestinal uptake of algae oil emulsions were investigated. It was found that the interfacial film formed by multi-modified β-Lg (EHG48β-Lg) at the water-oil interface resisted pepsin hydrolysis. In intestinal digestion, it was readily and effectively displaced by bile salts, which facilitated the adsorption of lipase. This effect endowed the algae oil emulsion stabilized by EHG48β-Lg with the highest free fatty acid release rate and a 329.2% increase in DHA bioaccessibility. Furthermore, compared with pure algae oil, the DHA uptake rate and apparent permeability coefficient of the EHG48β-Lg-stabilized algae oil emulsion increased by 54.4% and 85.0%, respectively. These results demonstrated that EHG48β-Lg can significantly improve the delivery and absorption efficiency of DHA by optimizing the interfacial structure of the emulsion.
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