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Measurement of polyphenol oxidase activity using optical waveguide lightmode spectroscopy-based immunosensor.
1Research Group of Convergence Technology, Korea Food Research Institute, Seongnam 463-746, Republic of Korea.
Food Chemistry
|September 20, 2014
Summary
A novel optical waveguide lightmode spectroscopy immunosensor offers sensitive and accurate measurement of polyphenol oxidase (PPO) activity. This PPO detection method is applicable to various food samples and demonstrates good reusability.
Area of Science:
- Food Science
- Biotechnology
- Analytical Chemistry
Background:
- Polyphenol oxidase (PPO) is a key enzyme impacting food quality during processing.
- Accurate and sensitive determination of PPO activity is crucial for the food industry.
Purpose of the Study:
- To develop a novel immunosensor for sensitive and rapid PPO activity measurement.
- To validate the sensor's performance with purified PPO and real food samples.
Main Methods:
- Development of an optical waveguide lightmode spectroscopy-based immunosensor.
- Immobilization of anti-PPO antibodies onto an optical grating coupler.
- Analysis of purified PPO and real food samples (potato, grape, Kimchi cabbage).
Main Results:
- A linear correlation was observed between sensor response and PPO activity (0.0005607-560.7U/mL).
- The sensor successfully measured PPO activity in potato, grape, and Kimchi cabbage samples.
- Analytical results correlated well with a conventional colorimetric assay.
Conclusions:
- The developed immunosensor provides a sensitive and reliable method for PPO activity determination.
- The sensor is applicable to diverse food matrices, aiding in food quality control.
- The sensor demonstrated good reusability for up to 10 days.
