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RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...

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Updated: Apr 23, 2026

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

Published on: May 23, 2018

BAC sequencing using pooled methods.

Christopher A Saski¹, F Alex Feltus, Laxmi Parida

¹Clemson University Genomics Institute, Clemson, SC, 29634, USA.

Methods in Molecular Biology (Clifton, N.J.)

|September 21, 2014

Summary

This summary is machine-generated.

Complex genome sequencing is challenging. This study presents a sub-genome sequencing protocol using bacterial artificial chromosomes (BACs) to reduce costs and improve assembly accuracy for targeted genomic regions.

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Area of Science:

Genomics
Bioinformatics
Molecular Biology

Background:

De novo genome sequencing of large, complex genomes is costly and difficult.
Challenges include repetitive DNA, paralogs, polyploidy, and heterozygosity, leading to fragmented assemblies.
Fragmented assemblies hinder biological interpretation.

Purpose of the Study:

To describe a protocol for sub-genome assembly.
To reduce sequencing costs and effort by targeting specific genomic regions.
To improve the accuracy of genome reconstruction.

Main Methods:

Utilizing bacterial artificial chromosome (BAC) sequencing.
Deriving a BAC tiling path from a genome-scale physical map or fine mapping.
Employing BAC isolation, mapping, DNA sequencing, and sequence assembly.

Main Results:

Sub-genomic sequencing reduces complexity.
Enables targeted exploration of prioritized genomic content.
Facilitates more accurate and less fragmented genome assemblies.

Conclusions:

Targeted sub-genome sequencing is a viable strategy for complex genomes.
BAC-based approaches offer a systematic method for cost-effective genome analysis.
This protocol aids in extracting meaningful biological insights from complex genomes.