On using magnesium and potassium ions in RNA experiments
1Department of Chemistry and Biochemistry, Colorado College, 14 E Cache La Poudre, Colorado Spring, CO, 80903, USA, NGrover@ColoradoCollege.edu.
Abstract:
RNA is a dynamic molecule that can adopt different conformations under different conditions. In the cell, the negatively charged RNA is expected to interact with potassium and magnesium ions. The role of magnesium ions has been extensively studied in RNA, as it is necessary for proper folding of RNA and acts as a nucleophile in many reactions catalyzed by RNA. A short overview of the role of magnesium and potassium in RNA structures is provided here in order to demonstrate the need for carefully choosing the experimental conditions for RNA studies.
More Related Videos
11:49A Novel Saturation Mutagenesis Approach: Single Step Characterization of Regulatory Protein Binding Sites in RNA Using Phosphorothioates
Published on: August 21, 2018
05:33Author Spotlight: Characterizing Novel Enzymes from Extremophiles and Common Pathogens to Understand DNA Repair and Replication
Published on: July 5, 2024
Related Concept Videos
Ribosomal RNA Synthesis
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
RNA Stability
