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Establishing pediatric reference intervals for 13 biochemical analytes derived from normal subjects in a pediatric

Sun-Mi Cho1, Sang-Guk Lee1, Ho Seong Kim2

  • 1Department of Laboratory Medicine, Severance Hospital, Yonsei University College of Medicine, Seoul, Republic of Korea.

Clinical Biochemistry
|September 23, 2014
PubMed

Insights

This study established updated age- and sex-specific reference intervals for 13 serum biochemical analytes in Korean children. These new pediatric reference intervals are crucial for accurate diagnosis and monitoring of children's health.

Area of Science:

  • Pediatric Endocrinology
  • Clinical Chemistry
  • Laboratory Medicine

Background:

  • Establishing accurate pediatric reference intervals is challenging due to physiological changes in children.
  • Variations in laboratory values can also be influenced by ethnic and behavioral factors.
  • Existing reference intervals may not reflect the specific characteristics of the Korean pediatric population.

Purpose of the Study:

  • To establish age- and sex-specific partitioned reference intervals for 13 key serum biochemical analytes in Korean children.
  • To provide updated and reliable laboratory data for pediatric healthcare providers.
  • To improve the accuracy of diagnostic interpretations in pediatric patients.

Main Methods:

  • Analysis of serum samples from 2474 healthy Korean children (girls aged 2-14, boys aged 2-16).
  • Measurement of 13 biochemical analytes including glucose, creatinine, and liver enzymes using a Hitachi 7600 analyzer.
  • Application of the Harris and Boyd method for partitioning reference intervals by age and sex.

Main Results:

  • Most analytes required partitioning by either age or sex, with few exceptions like calcium and albumin.
  • Age-specific intervals were established for alkaline phosphatase, creatinine, and total bilirubin in both sexes.
  • Sex-specific partitioning was sufficient for inorganic phosphorus, total cholesterol, alanine aminotransferase, blood urea nitrogen, and glucose.

Conclusions:

  • The study successfully generated updated age- and sex-specific pediatric reference intervals for 13 common serum chemistry analytes.
  • These findings are based on a large cohort of healthy Korean children utilizing modern analytical techniques.
  • The established intervals will aid in more precise clinical decision-making for pediatric patients.
Abstract

Keywords:
PartitioningPediatricReference interval

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