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[In vitro study of platelet preservation during 5 days in reduced-thickness bags]
J Domenech1, C Babault, I Desbois
1Centre Régional de Transfusion Sanguine de Tøurs.
Abstract:
An in vitro study of platelet concentrates storage for 5 days was performed in PVC bags. Modification of the original three-day containers were introduced by thickness reduction. The results at fifth day were comparable to those at third day in standard plastic bags. During storage, variations of platelet counts were very slight with a low LDH release. PH was stable with a good maintenance of phase microscope platelet morphology. PCO2 and PO2 measurements showed a satisfactory gas permeability which could explain a limited lactate production. If in vivo studies of transfusion recovery confirm these data, platelet concentrates storage could be extended up to 5 days by such modifications of standard three-day PVC bags.
Insights
Modified PVC bags allow for 5-day platelet concentrate storage, maintaining quality comparable to standard 3-day storage. This research suggests extended platelet viability with modified packaging.
Area of Science:
- Biomedical science
- Materials science
Context:
- Platelet concentrates are crucial for transfusions.
- Current storage limits are typically 3 days.
- Optimizing storage conditions is essential for blood banking.
Purpose:
- To evaluate the feasibility of extending platelet concentrate storage.
- To assess the impact of modified polyvinyl chloride (PVC) bags on platelet viability.
- To compare 5-day storage in modified bags with standard 3-day storage.
Summary:
- An in vitro study assessed platelet concentrate storage in modified PVC bags with reduced thickness for 5 days.
- Results showed platelet counts, pH, morphology, and gas permeability were comparable to standard 3-day storage.
- Low lactate dehydrogenase (LDH) release and stable pH indicate good platelet quality.
Impact:
- Modified PVC bags demonstrate potential for extending platelet concentrate shelf life to 5 days.
- This could improve blood product availability and reduce wastage.
- Further in vivo studies are needed to confirm transfusion recovery rates.