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Updated: Apr 23, 2026
Assessment of Vascular Tone Responsiveness using Isolated Mesenteric Arteries with a Focus on Modulation by Perivascular Adipose Tissues
Published on: June 3, 2019
[The measurement of the third-order branches of the mesenteric artery tone by microvascular ring technique]
Objective:
In our study, the function of the third-order branches of the mesentenc artery was measured by microvascular ring technique, which can be used to detect microvascular function in some disease related to microvascular dysfunction.
Methods:
Isolated, fixed, standardized and then activated the third-order branches of rat mesenteric artery. Microvascular tone was measured by systolic and diastolic drags respectively, with the help of DMT tension apparatus and PowerLab data acquisition system.
Results:
The third-order branches of rat mesenteric artery showed excellent response to vasoactive drugs. The contraction effect of norepinephrine (NE) reached 19 in mN. When acetylcholine (Ach) or sodium nitroprusside (SNP) of 10(9)-10(5)mol/L was added, vascular tones showed gradient drop: 80% of maximal relaxation when adding ACh, while 95% of maximal relaxation when adding SNP.
Conclusion:
The third-order branches of the mesenteric artery function was successfully detected by using microvascular ring technique.
Insights
The microvascular ring technique effectively measures mesenteric artery function. This method detects microvascular dysfunction in various diseases.
Area of Science:
- Physiology
- Vascular Biology
Context:
- Microvascular dysfunction is implicated in numerous diseases.
- Assessing the function of small blood vessels is crucial for understanding these conditions.
Purpose:
- To evaluate the efficacy of the microvascular ring technique for assessing the function of third-order branches of the rat mesenteric artery.
- To establish a reliable method for detecting microvascular function.
Summary:
- The study utilized the microvascular ring technique to measure the function of isolated rat mesenteric artery branches.
- Vascular tone responses to vasoactive drugs like norepinephrine, acetylcholine, and sodium nitroprusside were recorded.
- The technique demonstrated a robust ability to detect significant contractile and relaxant responses.
Impact:
- This research validates the microvascular ring technique as a valuable tool for studying microvascular function.
- The findings support its application in research related to microvascular dysfunction and related diseases.
