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Updated: Apr 23, 2026

Detection of Horizontal Gene Transfer Mediated by Natural Conjugative Plasmids in E. coli
Published on: March 24, 2023
blaNDM-5 carried by an IncX3 plasmid in Escherichia coli sequence type 167
Ping Yang1, Yi Xie2, Ping Feng3
1Center of Infectious Diseases, West China Hospital, Sichuan University, Chengdu, China Division of Infectious Diseases, State Key Laboratory of Biotherapy, Chengdu, China.
Abstract:
bla(NDM-)5 was found in Escherichia coli strain 0215 from a Chinese patient without travel history. Genomic sequencing and conjugation experiments were performed. Strain 0215 belonged to sequence type 167 (ST167) and had other resistance determinants, including bla(TEM-135), bla(CTX-M-14), and aac(6')-Ib. bla(NDM-5) was carried by a 47-kb self-transmissible IncX3 plasmid and was in a complex genetic context similar to that of bla(NDM-1) on IncX3 plasmids. IncX3 plasmids might have emerged as a common vehicle mediating the spread of bla(NDM).
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