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Updated: Apr 23, 2026

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis
Published on: July 8, 2025
Invasive reaction assisted strand-displacement signal amplification for sensitive DNA detection
Bingjie Zou1, Qinxin Song, Jianping Wang
1Department of Pharmacology, Jinling Hospital, Medical School of Nanjing University, Nanjing, 210002, China. ghzhou@nju.edu.cn.
Abstract:
A novel DNA detection assay was proposed by invasive reaction coupled with molecular beacon assisted strand-displacement signal amplification (IRASA). Target DNAs are firstly hybridized to two probes to initiate invasive reaction to produce amplified flaps. Then these flaps are further amplified by strand-displacement signal amplification. The detection limit was around 0.2 pM.
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