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Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

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Updated: Apr 23, 2026

Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy

Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy

Published on: May 16, 2022

Upconversion nanoparticles: a versatile solution to multiscale biological imaging.

Xiang Wu¹, Guanying Chen, Jie Shen

¹Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School , Worcester, Massachusetts 01605, United States.

Bioconjugate Chemistry

|September 26, 2014

Summary

This summary is machine-generated.

Lanthanide-doped photon upconverting nanomaterials offer advanced biomedical imaging capabilities. These upconverting nanoparticles (UCNPs) overcome limitations of conventional probes for deep tissue imaging and therapy.

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Area of Science:

Biomedical Imaging
Nanotechnology
Materials Science

Background:

Conventional fluorescent probes face limitations in deep tissue imaging due to high background and shallow light penetration.
Lanthanide-doped photon upconverting nanomaterials offer unique optical properties for overcoming these challenges.

Purpose of the Study:

To review the limitations of conventional fluorescent probes.
To highlight the advancements of upconverting nanoparticles (UCNPs) for multiscale biological imaging.
To present UCNPs as a promising alternative for enhanced biomedical applications.

Main Methods:

Literature review focusing on upconverting nanoparticles (UCNPs).
Analysis of UCNP properties including photon upconversion, low background, large anti-Stokes shift, and high optical penetration depth.
Comparison of UCNP performance against conventional imaging probes.

Main Results:

Upconverting nanoparticles (UCNPs) efficiently convert near-infrared light to shorter wavelengths, enabling deeper tissue penetration.
UCNPs exhibit low imaging background and a large anti-Stokes shift, improving signal-to-noise ratio.
These properties facilitate applications in deep tissue optical imaging, drug delivery, and photothermal therapy.

Conclusions:

Upconverting nanoparticles (UCNPs) represent a significant advancement over traditional fluorescent probes for biomedical imaging.
Their unique optical characteristics provide solutions for multiscale biological imaging and targeted therapies.
UCNPs are poised to revolutionize various aspects of medical diagnostics and treatment.