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Entry of Bombyx mori nucleopolyhedrovirus into BmN cells by cholesterol-dependent macropinocytic endocytosis

Jinshan Huang1, Bifang Hao1, Chen Cheng2

  • 1College of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, Jiangsu 212018, PR China; Sericultural Research Institute, Chinese Academy of Agricultural Science, Zhenjiang, Jiangsu 212018, PR China.

Biochemical and Biophysical Research Communications
|September 30, 2014
PubMed

Insights

Bombyx mori nucleopolyhedrovirus (BmNPV) infection in silkworms lacks specific treatments. This study reveals BmNPV enters cells via cholesterol-dependent macropinocytosis, offering potential targets for protection.

Area of Science:

  • Virology
  • Cell Biology
  • Insect Pathology

Background:

  • Bombyx mori nucleopolyhedrovirus (BmNPV) is a significant pathogen affecting silkworm production.
  • Current treatments and protective measures against BmNPV are unavailable.
  • Understanding BmNPV cell entry mechanisms is crucial for developing control strategies.

Purpose of the Study:

  • To elucidate the mechanism by which BmNPV enters insect cells.
  • To identify key cellular processes and molecules involved in BmNPV entry.

Main Methods:

  • Application of various endocytic inhibitors to BmN cells.
  • Subcellular localization analysis of BmNPV during entry.
  • Dose-dependent assessment of cholesterol involvement.

Main Results:

  • BmNPV entry is mediated by clathrin-independent macropinocytic endocytosis.
  • Cholesterol plays a critical role in BmNPV cell entry in a dose-dependent manner.
  • Partial rescue of BmNPV infection was observed upon cholesterol replenishment.

Conclusions:

  • BmNPV utilizes a specific cholesterol-dependent macropinocytosis pathway for cell entry.
  • Targeting cholesterol-mediated pathways could offer a novel approach for BmNPV control in silkworms.
Keywords:
BmNPVCholesterolEndocytosisMacropinocytosis

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