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Immunohistochemical expression of perforin in lichen planus lesions
Mohamed Abdelwahed Gaber1, Alaa Hassan Maraee, Dalia Rifaat Alsheraky
1Department of Dermatology and Andrology, Faculty of Medicine, Menoufia University , Shebeen-Elkoum , Egypt .
Background:
Lichen planus (LP) is a chronic inflammatory papulosquamous skin disease characterized by epidermal basal cell damage and a particular band-like infiltrate predominantly of T cells in the upper dermis. It is characterized by the formation of colloid bodies representing apoptotic keratinocytes. The apoptotic process mediated by CD8+ cytotoxic T lymphocytes and natural killer cells mainly involves two distinct pathways: the perforin/granzyme pathway and the Fas/FasL pathway. So far, little is known regarding the role of perforin-mediated apoptosis in LP.
Aim:
Is to study the expression and distribution of perforin in the epidermis and dermis of lesional LP skin.
Materials And Methods:
Skin biopsy specimens from lesional skin of 31 patients with LP and 10 healthy persons were analyzed by immunohistochemistry.
Results:
Significant accumulation of perforin + cells was found in both epidermis and dermis of LP lesions compared with healthy skin. Perforin expression was significantly upregulated in the epidermis of LP lesions.
Conclusion:
Accumulation of perforin + cells in the epidermis of LP lesions suggest a potential role of perforin in the apoptosis of basal keratinocytes.
