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Related Concept Videos

Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

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Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
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Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
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Related Experiment Videos

Multiplexed, quantitative, and targeted metabolite profiling by LC-MS/MRM.

Ru Wei1, Guodong Li, Albert B Seymour

  • 1Proteomics, Translational Science, Biogen Idec, 14 Cambridge Center, Cambridge, MA, 02142, USA, ru.wei@biogenidec.com.

Methods in Molecular Biology (Clifton, N.J.)
|October 2, 2014
PubMed
Summary
This summary is machine-generated.

Targeted metabolomics enables biomarker discovery by analyzing known metabolites. This study presents a rapid method to quantify 205 endogenous metabolites using multiple reaction monitoring (MRM) in just 10 minutes.

Related Experiment Videos

Area of Science:

  • Metabolomics
  • Biochemistry
  • Analytical Chemistry

Background:

  • Targeted metabolomics identifies biomarkers and links disease to mechanisms.
  • Quantitative measurements of known metabolites offer immediate biological insights.
  • Analyzing diverse endogenous metabolites simultaneously poses significant challenges.

Purpose of the Study:

  • To develop a rapid and comprehensive method for quantitative profiling of endogenous metabolites.
  • To overcome the challenges of analyzing a large number of metabolites with diverse properties.

Main Methods:

  • Combined chromatographic separation conditions.
  • Optimized ionization polarities.
  • Utilized triple-quadrupole MS with multiple reaction monitoring (MRM) for sensitive detection.

Main Results:

  • Successfully developed a method for quantitative profiling.
  • Analyzed 205 endogenous metabolites.
  • Achieved quantitative profiling within a 10-minute timeframe.

Conclusions:

  • The developed method enables rapid and quantitative analysis of a substantial number of endogenous metabolites.
  • This approach enhances the utility of targeted metabolomics for biomarker discovery and mechanistic studies.