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Updated: Apr 23, 2026

Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry
Published on: July 12, 2013
Analysis of dried blood spots using DESI mass spectrometry
Justin M Wiseman1, Joseph H Kennedy
1Prosolia, Inc., 351 West 10th Street, Indianapolis, IN, 46202, USA, wiseman@prosolia.com.
Abstract:
Dried blood spot (DBS) analysis using mass spectrometry is an invaluable technique for examining blood markers of inborn metabolic diseases in clinical laboratories. Implementation of DBS sampling and analysis in pharmaceutical development have more recently gained traction due to the advantages of convenience in sample procurement and logistics, as well as the innate advantages associated with the collection of lower blood volumes. While there are several realized advantages of DBS, the bioanalytical laboratory is disadvantaged and burdened with additional preparative steps prior to analysis. Therefore, improvements in the laboratory workflow for DBS analysis are necessary. Here, we describe direct blood spot analysis using desorption electrospray ionization (DESI) mass spectrometry for quantitative determination of drugs in whole blood.
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