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Related Experiment Video

Updated: Apr 23, 2026

A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells
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Antioxidative properties of ascorbigen in using multiple antioxidant assays.

Akihiro Tai1, Kanako Fukunaga, Asako Ohno

  • 1a Faculty of Life and Environmental Sciences , Prefectural University of Hiroshima , Shobara , Japan.

Bioscience, Biotechnology, and Biochemistry
|October 3, 2014
PubMed
Summary

Ascorbigen, found in Brassica vegetables, exhibits potent antioxidant activity, outperforming ascorbic acid and Trolox in key assays. This compound may offer significant health benefits beyond previously understood levels.

Keywords:
ABTS radical cationORACantioxidantascorbigenoxidative hemolysis inhibition assay

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Area of Science:

  • Biochemistry
  • Nutritional Science
  • Food Chemistry

Background:

  • Ascorbigen is a major indole-derived compound in Brassica vegetables.
  • Antioxidants play a crucial role in combating oxidative stress.
  • Understanding the antioxidant capacity of dietary compounds is vital for health.

Purpose of the Study:

  • To systematically evaluate the antioxidative properties of ascorbigen.
  • To compare ascorbigen's antioxidant activity against ascorbic acid and Trolox.
  • To elucidate the mechanisms underlying ascorbigen's radical scavenging capabilities.

Main Methods:

  • Utilized multiple assay systems including DPPH, galvinoxyl, and ABTS radical scavenging assays.
  • Assessed antioxidant capacity using Oxygen Radical Absorbance Capacity (ORAC) assay.
  • Evaluated oxidative hemolysis inhibition assay with AAPH-derived peroxyl radicals.

Main Results:

  • Ascorbigen demonstrated superior ABTS radical cation scavenging compared to ascorbic acid.
  • The indole moiety of ascorbigen was identified as a key contributor to radical scavenging.
  • Ascorbigen exhibited significantly stronger antioxidant activity than ascorbic acid and Trolox in ORAC and hemolysis assays.

Conclusions:

  • Ascorbigen possesses potent antioxidative properties, particularly against physiologically relevant radicals.
  • Its efficacy surpasses that of well-known antioxidants like ascorbic acid and Trolox.
  • Ascorbigen's antioxidant activity suggests potential benefits for daily health care.