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Purification of mitochondria by sucrose step density gradient centrifugation
David A Clayton1, Gerald S Shadel2
1Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia 20147-2408;
Cold Spring Harbor Protocols
|October 3, 2014
Abstract:
Mitochondrial fractions isolated from tissue culture cells or tissue such as liver after differential centrifugation can be purified further by density gradient centrifugation. Here we describe the use of sucrose for this purpose because it is commonly used and inexpensive and the resulting mitochondria preparations are useful for many purposes.

